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where A test sample is the absorbance of the test sample and A control is the absorbance of the control. 2.11. In vitro cytotoxicity HepG2 cells, at a density of 1 × 10 6 cells per cm 2, were washed and trypsinized after incubation with SPIO or SPIO-HA-DOX NPs, respectively, at Fe concentrations of 40 μg mL −1 in serum-free DMEM medium at 37 °C for 24 h. . Cells were then mixed with 2%
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